FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102354
Interaction Network
Interactions Browser links
CG7483 network
mago network
Reported Interactions
FBrf0174990-2.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4AIII

bait

GST tag

mago 

Mago

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0225497-14.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4AIII

bait

HA tag

mago 

Mago

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The eIF4AIII-mago interaction is disrupted by coexpression of Pym (N-terminal 60 residues is sufficient).

FBrf0225497-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4AIII

bait
mago 

Mago

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The eIF4AIII-mago interaction is disrupted by overexpression of transgenic Pym.

FBrf0225497-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mago 

Mago

bait

GFP tag

eIF4AIII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The eIF4AIII-mago interaction is disrupted by overexpression of transgenic Pym (N-terminal 60 residues is sufficient).

FBrf0243074-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mago 

Mago

bait

GFP tag

eIF4AIII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0243074-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mago 

Mago

bait

GFP tag

eIF4AIII

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene or tagged transgenic construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)