Spag
FLAG tag
CG4147
12 peptides identified
Source was soluble cytosolic extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Affinity purification of bait and identification of prey by MALDI peptide mapping.
GST tag
HSC70-3
[35]S label
\'Δ2Ct\'
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
The spag TPR motifs are necessary for Hsc70-4 binding, while the spag C-terminus is necessary for Hsc70-3 binding.
