FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
MED19, MED1
FlyBase ID
FBig0000102364
Interaction Network
Interactions Browser links
MED19 network
MED1 network
Reported Interactions
FBrf0224861-18.coIP.WB
Reference
Boube et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

Venus tag, C-terminal fragment

MED19 
MED1 

Ubx

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
MED19 
homeodomain-interacting motif (HIM)
necessary binding region
aa 160-229

\'ΔHIM\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Boube et al., 2014)

Deletion of the MED19 homeodomain-interacting motif (HIM) does not disrupt the MED19-MED1 interaction.

(Boube et al., 2014)

Positive control.

(Boube et al., 2014)
FBrf0246810-10.PD.A
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

GST tag

MED1 

Med1

prey

[35]S label

MED1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
MED1 
N-terminal domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Immarigeon et al., 2020)
FBrf0246810-9.PD.A
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MED1 

Med1

bait

GST tag

MED19 

Med19

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Immarigeon et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)