FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
imd, Usp2
FlyBase ID
FBig0000102410
Interaction Network
Interactions Browser links
imd network
Usp2 network
Reported Interactions
FBrf0225968-1.coIP.WB
Reference
Engel et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
imd 

Imd

bait

V5 tag

imd 
imd 
Usp2 

USP2

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
imd 
N-terminal region
sufficient binding region
aa 1-178
imd 
N-terminal region
necessary binding region
aa 1-176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Engel et al., 2014)

Co-transfection of Usp2 with imd leads to reduced levels of imd-linked K48 ubiquitin (but does not affect imd-linked K63 ubiquitin levels).

(Engel et al., 2014)
FBrf0225968-2.coIP.WB
Reference
Engel et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Usp2 

USP2

bait

Myc tag

imd 

Imd

prey

V5 tag

imd 
imd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
imd 
N-terminal region
sufficient binding region
aa 1-178
imd 
N-terminal region
necessary binding region
aa 1-176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Engel et al., 2014)
FBrf0225968-3.PD.WB
Reference
Engel et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Usp2 

USP2

bait

GST tag

Usp2 
imd 

Imd

prey

V5 tag

imd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp2 
N-terminal region
sufficient binding region
aa 1-531
imd 
N-terminal region
necessary binding region
aa 1-474
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Engel et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)