FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ac, Act5C
FlyBase ID
FBig0000102494
Interaction Network
Interactions Browser links
ac network
Act5C network
Reported Interactions
FBrf0223751-1.coIP.MS
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ac 

Ac

bait

Myc tag

Act5C 

Actin

prey

12 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hsiao et al., 2014)

Act5C coimmunoprecipitates with ac in nuclear, but not cytosolic, extracts.

(Hsiao et al., 2014)
FBrf0223751-3.coIP.WB
Reference
Hsiao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ac 

Ac

bait

Myc tag

Act5C 

Actin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hsiao et al., 2014)

Actin coimmunoprecipitates with ac in nuclear, but not cytosolic, extracts.

(Hsiao et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)