Source was cell extract of 293T cell line; enzyme produced from transfected construct.
Lrrk autophosphorylates in vitro.
Lrrk was immunopurified from transfected 293T cells and incubated in the presence of radiolabelled [32]P-ATP. Autophosphorylation was assessed by gel electrophoresis and autoradiography.
Interaction in vitro; enzyme immunopurified from transfected 293T cell extract.
Source was adult heads of transgenic fly line; protein produced from tagged transgenic construct.
Lrrk forms dimers and higher order oligomers.
