Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

elav binds ultraconserved "elav binding sequences" EBS3 and EBS8 in Ubx mRNA (binds weakly to EBS13 and EBS16 also).

Radiolabeled RNA and purified protein were UV cross-linked, treated with RNaseA and protected, labeled RNA was visualized by gel electrophoresis and autoradiography.