Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

GST pulldown experiments could not detect binding between east and Chro. The authors also tried to determine the east interacting domain of Chro however none of the constructs containing residues 437–678, 555–803 or 678–927 of Chro showed binding to east.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Two-hybrid system: yeast GAL4-BD/GAL4-AD