FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smn, Actn
FlyBase ID
FBig0000102915
Interaction Network
Interactions Browser links
Smn network
Actn network
Reported Interactions
FBrf0194681-1.coIP.MS
Reference
Rajendra et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Smn 

dSMN

bait

FLAG tag

Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rajendra et al., 2007)

Flag-Smn was transiently transfected into S2 cells and purified over anti-FLAG beads. Eluted proteins were subjected to SDS-PAGE and Coomassie staining. Bands were excised from the gel and identified by mass spectrometry as Actn, Flag-Smn, and Smn.

(Rajendra et al., 2007)
FBrf0194681-2.PD.WB
Reference
Rajendra et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

dSMN

bait

GST tag

Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type total adult extract.

(Rajendra et al., 2007)
FBrf0194681-3.coIP.WB
Reference
Rajendra et al., 2007
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smn 

SMN

bait
Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoraces of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Rajendra et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)