FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lasp, Actn
FlyBase ID
FBig0000102965
Interaction Network
Interactions Browser links
Lasp network
Actn network
Reported Interactions
FBrf0225918-1.TAP.WB
Reference
Fernandes and Schöck, 2014
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lasp 

Lasp

bait

His tag; FLAG tag

Lasp 
Lasp 
Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lasp 
Lim domain
necessary binding region
aa 4-100

coordinates relative to Lasp-PB

Lasp 
nebulin repeats
necessary binding region
aa 106-171

coordinates relative to Lasp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was thoraces of adult of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fernandes and Schöck, 2014)

Deletion of the SH3 domain of Lasp, or mutations resulting in amino acid changes from Y to S at aa 86 or aa 121 of Lasp, do not interfere with Actn binding.

(Fernandes and Schöck, 2014)
FBrf0225918-15.coIP.MS
Reference
Fernandes and Schöck, 2014
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lasp 

Lasp

bait

GFP tag

Actn 

α-Actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoraces of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fernandes and Schöck, 2014)

Actn was enriched in Lasp-GFP coimmunoprecipitation compared to GFP-only coimmunoprecipitation.

(Fernandes and Schöck, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)