FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, pAbp
FlyBase ID
FBig0000103047
Interaction Network
Interactions Browser links
pum network
pAbp network
Reported Interactions
FBrf0225676-4.PD.WB
Reference
Weidmann et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pum 

PUM

bait

Halotag

pum 
pAbp 

pAbp

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RNA-binding domain
sufficient binding region
aa 1091-1427

coordinates relative to 1533 aa isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Weidmann et al., 2014)

Halotag pulldown, TEV elution and Myc Western blot detection of prey.

(Weidmann et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)