FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103133
Interaction Network
Interactions Browser links
His2A network
His3 network
Reported Interactions
FBrf0084814-6.CS.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

core histone

neutral component

core histone

neutral component

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins purified from embryonic nuclear extract.

FBrf0087451-6.CS.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

core histone

neutral component

core histone

neutral component

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins purified from embryonic nuclear extract.

FBrf0090625-6.CS.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

core histone

neutral component

core histone

neutral component

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
His3 

core histone

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins purified from embryonic nuclear extract.

FBrf0225785-2.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

H2A

neutral component

H2A

neutral component

H2A

neutral component
His3 

H3

neutral component
His3 

H3

neutral component
His3 

H3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Recombinant histones were separately expressed and purified under denaturing conditions to directly solubilize inclusion bodies, then mixed (1mg each) and purified by size exclusion chromatography.

Recombinant His2A, His2B, His3 and His4 could be purified as a histone octamer complex.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0232109-2.CS.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

H2A

neutral component

H2A

neutral component

H2A

neutral component
His3 

H3

neutral component
His3 

H3

neutral component
His3 

H3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (5)