FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Taf4, Taf12
FlyBase ID
FBig0000103234
Interaction Network
Interactions Browser links
Taf4 network
Taf12 network
Reported Interactions
FBrf0065619-16.coIP.A
Reference
Yokomori et al., 1993
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf12 

dTAFII30α

bait

HA tag

Taf4 

dTAFII110

prey

[35]S label

Taf4 
Taf4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf4 
C-terminal region
sufficient binding region
aa 572-921

coordinates relative to Taf4-PA

Taf4 
C-terminal region
necessary binding region
aa 852-921

coordinates relative to Taf4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Yokomori et al., 1993)

Taf12 coimmunoprecipitates Taf4 but not Taf11 or Taf6.

(Yokomori et al., 1993)
FBrf0065619-17.coIP.A
Reference
Yokomori et al., 1993
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf4 

dTAFII110

bait

HA tag

Taf12 

dTAFII30α

prey

[35]S label

Taf12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf12 
C-terminal region
sufficient binding region
aa 49-196
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey produced and labeled by in vitro translation.

(Yokomori et al., 1993)
FBrf0180166-2.PD.WB
Reference
Hiller et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf4 

dTAF4

bait

GST tag

Taf12 

dTAF12

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Hiller et al., 2004)

Taf4 and Taf12 interact with each other, but not with testis-specific homologs nht (Taf4L) or Taf12L in this assay.

(Hiller et al., 2004)

Positive control.

(Hiller et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)