Rho-4
Spi
GFP tag
DmRho4
GST tag
fused to GFP extracellular domain with cytosolic FLAG tag
fused to Hsap\APP extracellular domain with cytosolic FLAG tag
FLAG tag
Spitz
The coimmunoprecipitation of spi with rho-4 is not affected by treatment with Ca2+ ionophore. Also, there is no evidence for rho-4 homodimerization in the presence or absence of Ca2+.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
A catalytically inactive from of rho-4 was used in this assay.
