Positive control.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait oligonucleotides synthesized in vitro; prey derived from clone 8 cell extract.
The authors use biotin-tagged oligonucleotides complementary to roX1 to pull down associated factors (protein, RNA or DNA) from cross-linked chromatin.