Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

Sxl binds to the non-sex-specific intron of tra, blocking splicing at this site and promoting usage of the less active female-specific splice site upstream.

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

Sxl and U2af65 compete for binding to the non-sex-specific intron of tra. Binding of Sxl to the non-sex-specific intron blocks U2AF and promotes usage of the lower affinity female-specific splice site upstream.

RNA-bound protein was UV crosslinked, separated by SDS-PAGE and the molecular weight of the bound protein was visualized by autoradiography.