Source was fixed larval body wall of wild-type fly line; proteins produced from endogenous genes.

hts also binds phosphoinositides via it\'s MARCKS domain (GSPKKDKKKKKGLRTPSFLKKKKEKKKAEA).

The hts antibody used recognizes the C-terminal MARCKS domain. The dlg1 antibody used recognizes the N-terminal PDZ domain. The PLA signal develops only if both primary antibodies are in close proximity to each other (within tens of nanometers).

Source was fixed larval body wall of wild-type fly line; proteins produced from endogenous genes.

The hts antibody used recognizes the C-terminal MARCKS domain. The dlg1 antibody used recognizes the N-terminal PDZ domain. The PLA signal develops only if both primary antibodies are in close proximity to each other (within tens of nanometers).