FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nedd4, N
FlyBase ID
FBig0000103450
Interaction Network
Interactions Browser links
Nedd4 network
N network
Reported Interactions
FBrf0183817-1.coIP.WB
Reference
Sakata et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

Nedd4

bait

HA tag

N 

Notch

prey

FLAG tag

N 
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
cytoplasmic domain
sufficient binding region
N 
WW-binding motif
mutation disrupting interaction
aa 2328

Y2328F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sakata et al., 2004)
FBrf0183817-2.PD.WB
Reference
Sakata et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

Nedd4

bait

His tag

Nedd4 
Nedd4 
N 

N

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
cytoplasmic domain
sufficient binding region
Nedd4 
WW1 domain
sufficient binding region
aa 181-343

weaker binding than WW2-3

Nedd4 
WW2 and WW3 domains
sufficient binding region
aa 344-452

stronger binding than WW1

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Sakata et al., 2004)

RNAi knockdown of Nedd4 in S2 cells diminishes the level of polyubiquitinated N.

(Sakata et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)