Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey produced as a recombinant fusion protein in baculovirus-Sf9 cell system.
V43E, not V43Q ("mut4")
K46V, Q47A ("mut6")
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Positive control.
