Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

E(spl)m7-HLH interacts with ac, sc, ase, da and gro, but not with E(spl)m3-HLH, in this assay.

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast lexA-BD/B42-AD

Source was S2 cell line; bait produced as transgenic fusion protein; prey produced from transfected construct (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was S2 cell line; bait produced as transgenic fusion protein; prey produced from transfected construct (prey was previously cloned reagent).

A transcriptional activator fragment of da was fused to a GAL4 DNA binding domain and shown to drive expression from a UAS luciferase reporter. Interaction inferred from ability of coexpressed proteins to inhibit da dependent reporter activation.