Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

Short range cooperative TF-TF interactions predicted from the analysis of multiple ChIP data sets were tested by in vitro pull down assay. Assays showing greater then 7-fold enrichment over negative controls were considered to be validated.

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

Short range cooperative TF-TF interactions predicted from the analysis of multiple ChIP data sets were tested by in vitro pull down assay. Assays showing greater then 7-fold enrichment over negative controls were considered to be validated.