Ptc
fused to GAL4 DNA-binding domain
Su(dx)
fused to GAL4 activation domain
"CTD"
PPAY to AAAA
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
All three "C2-WW-HECT" E3 family proteins can bind to the C-terminal domain of ptc : Su(dx), Nedd4 and Smurf.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Myc tag
FLAG tag
Treatment of cells with hh conditioned medium or depletionof smo increased the ptc-Su(dx) interaction.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
