FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Alk, jeb
FlyBase ID
FBig0000103664
Interaction Network
Interactions Browser links
Alk network
jeb network
Reported Interactions
FBrf0167923-1.coIP.WB
Reference
Englund et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alk 

Alk

bait

HA tag

Alk 
jeb 

Jeb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Alk 
extracellular domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type or transgenic fly line; bait produced from endogenous gene or transgenic construct; prey produced from endogenous gene.

(Englund et al., 2003)
FBrf0167923-2.AF.FM
Reference
Englund et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alk 

Alk

bait
jeb 

Jeb

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was COS7 cells; bait produced from transfected construct; prey produced as a recombinant fusion protein in bacterial system.

(Englund et al., 2003)

jeb binding specifically to cells expressing Alk at the cell surface was taken as evidence of a jeb-Alk interaction.

(Englund et al., 2003)
FBrf0167923-3.ELISA
Reference
Englund et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
jeb 

Jeb

bait
Alk 

Alk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey source is unspecified.

(Englund et al., 2003)
FBrf0173097-1.AT.TV
Reference
Lee et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Alk 

Alk

bait
jeb 

Jeb

prey

AP tag

jeb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
jeb 
type-A LDL receptor repeat
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait present on live, transfected mammalian 293T cells; prey produced and labeled by in vitro translation.

(Lee et al., 2003)

jeb binds Alk with Kd = 2.2 nM.

(Lee et al., 2003)

The amount of AP-tagged jeb binding to the surface of Alk-expressing cells was determined by alkaline phosphatase (AP) activity.

(Lee et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)