FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
tkv, fu
FlyBase ID
FBig0000103669
Interaction Network
Interactions Browser links
tkv network
fu network
Reported Interactions
FBrf0212535-1.coIP.MS
Reference
Xia et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

FLAG tag

fu 

Fu

prey

2 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xia et al., 2010)

Cells were treated with MG132 to inhibit protein degradation and enrich for factors involved in tkv protein degradation.

(Xia et al., 2010)
FBrf0212535-2.coIP.WB
Reference
Xia et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
fu 

Fu

bait

Myc tag

fu 
fu 
tkv 

Tkv

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fu 
N-terminal region
sufficient binding region
fu 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xia et al., 2010)

The N- and C-terminal regions of fu can each bind tkv.

(Xia et al., 2010)
FBrf0212535-3.coIP.WB
Reference
Xia et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

FLAG tag

tkv 
fu 

Fu

prey

Myc tag

fu 
fu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tkv 
STYKc domain
sufficient binding region
fu 
N-terminal region
sufficient binding region
fu 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xia et al., 2010)

The N-terminal region of fu binds the STYKc domain of tkv. The C-terminal region of fu can separately bind both STYKc and ΔSTYKc tkv constructs.

(Xia et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)