FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hem, Abi
FlyBase ID
FBig0000103733
Interaction Network
Interactions Browser links
Hem network
Abi network
Reported Interactions
FBrf0162072-1.Y2H
Reference
Bogdan and Klambt, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hem 

Kette

bait

fused to GAL4 DNA-binding domain

Abi 

Abi

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan and Klambt, 2003)

Abi interacts with Hem (kette) and WASp, but no direct interaction between Hem and WASp was observed.

(Bogdan and Klambt, 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bogdan and Klambt, 2003)
FBrf0188146-2.Y2H
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

unspecified role
Abi 

Abi

unspecified role
Abi 

Abi

unspecified role
Hem 

Kette

unspecified role
Hem 

Kette

unspecified role
Hem 

Kette

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan et al., 2005)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bogdan et al., 2005)
FBrf0216499-3.CH.WB
Reference
Stephan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

neutral component
Abi 

Abi

neutral component
Abi 

Abi

neutral component
Hem 

Kette

neutral component
Hem 

Kette

neutral component
Hem 

Kette

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from adult head extract.

(Stephan et al., 2011)

A large fraction of Abi cofractionates with SCAR and the WAVE complex subunit Hem in protein complexes of about 400-500 kDa.

(Stephan et al., 2011)
FBrf0242076-2.PD.WB
Reference
Kim et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

GST tag

Abi 
Abi 
Hem 

Kette

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abi 
interaction domain
sufficient binding region
aa 1-387

coordinates relative to Abi-PA

Abi 
HHR domain
necessary binding region
aa 123-175

coordinates relative to Abi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Kim et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)