FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
grnd, Traf6
FlyBase ID
FBig0000103749
Interaction Network
Interactions Browser links
grnd network
Traf6 network
Reported Interactions
FBrf0228803-1.coIP.WB
Reference
Andersen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
grnd 

Grnd

bait

FLAG tag

grnd 
grnd 
Traf6 

Traf2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grnd 
Traf6-binding motif residue 202
mutation disrupting interaction
aa 202

E202A

grnd 
transmembrane and intracellular domain
sufficient binding region
aa 99-241

"intra" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Andersen et al., 2015)
FBrf0228803-3.PD.MW
Reference
Andersen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grnd 

Grnd

bait

GST tag

grnd 
grnd 
Traf6 

Traf2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grnd 
Traf6-binding motif residue 202
mutation disrupting interaction
aa 202

E202A

grnd 
transmembrane and intracellular domain
sufficient binding region
aa 99-241

"intra" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Andersen et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)