Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Positive control.
L501P or L501Q, coordinates relative to Axn-PA
coordinates relative to Axn-PA
coordinates relative to Axn-PA
H504L or H504R or L501Q, coordinates relative to Axn-PA
coordinates relative to Axn-PA
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Saturating mutagenesis of Axn prey to identify mutations that disrupt binding. Western blotting eliminated lost interaction due to nonsense mutations. Since clones contained, on average, 3 mutation each, single point mutations were subsequently tesetd.
