FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Axn, arr
FlyBase ID
FBig0000103776
Interaction Network
Interactions Browser links
Axn network
arr network
Reported Interactions
FBrf0158734-2.PD.WB
Reference
Yamazaki and Yanagawa, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arr 

Arrow

bait

GST tag

arr 
Axn 

Axin

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arr 
cytoplasmic tail
sufficient binding region
aa 1185-1393
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey produced as a recombinant fusion protein in baculovirus-Sf9 cell system.

(Yamazaki and Yanagawa, 2003)
FBrf0158859-1.Y2H
Reference
Tolwinski et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arr 

Arrow

bait

fused to GAL4 DNA-binding domain

arr 
Axn 

Axin

prey

fused to GAL4 activation domain

Axn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arr 
cytoplasmic domain
sufficient binding region
aa 1472-1678
Axn 
C-terminal region
sufficient binding region
aa 418-745
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Tolwinski et al., 2003)

15 Axn clones were recovered using the arr cytoplasmic domain as bait.

(Tolwinski et al., 2003)

The Axn N-terminal region inhibits the Axn-arr interaction.

(Tolwinski et al., 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Tolwinski et al., 2003)
FBrf0238042-2.coIP.WB
Reference
Tacchelly-Benites et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Axn 

Axin

bait

V5 tag

arr 

Arrow

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Phosphorylated arr was detected in Axn precipitates. Deletion of the Apc binding domain of Axn decreased the Axn-phospho-arr interaction suggesting that Apc normally promotes this interaction.

(Tacchelly-Benites et al., 2018)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Tacchelly-Benites et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)