FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Chi, pygo
FlyBase ID
FBig0000103799
Interaction Network
Interactions Browser links
Chi network
pygo network
Reported Interactions
FBrf0229654-2.NMR
Reference
Fiedler et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

Pygo

neutral component

15[N] label

pygo 

Pygo

neutral component

15[N] label

pygo 
pygo 
pygo 

Pygo

neutral component

15[N] label

pygo 
pygo 
Chi 

Chip-SSDP

neutral component
Chi 
Chi 

Chip-SSDP

neutral component
Chi 

Chip-SSDP

neutral component
Chi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
NPF motif
mutation disrupting interaction
aa 99

F99A

pygo 
interaction domain
sufficient binding region
aa 67-107
Chi 
interaction domain
sufficient binding region
aa 205-436
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Chi protein aa 205-436 co-expressed with Ssdp aa 1-92. Abbreviated as ChiLS or Chip-SSDP in publication.

(Fiedler et al., 2015)

Chi aa 205-436 requires the presence of Ssdp aa 1-92 in order to bind pygo.

(Fiedler et al., 2015)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Fiedler et al., 2015)
FBrf0229654-6.NMR
Reference
Fiedler et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chi 

Chip

bait
pygo 

Pygo

prey

15[N] label

pygo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
interaction domain
sufficient binding region
aa 67-107
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Fiedler et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)