FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Kap-α1, tim
FlyBase ID
FBig0000103816
Interaction Network
Interactions Browser links
Kap-α1 network
tim network
Reported Interactions
FBrf0227552-4.coIP.WB
Reference
Jang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Kap-α1 

imp-α1

bait

VSV tag

Kap-α1 
tim 

tim

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kap-α1 
IBB domain
mutation increasing interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jang et al., 2015)

Binding of Kap-α1 to tim requires mutation in IBB domain of Kap-α1.

(Jang et al., 2015)
FBrf0227552-5.coIP.WB
Reference
Jang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Kap-α1 

imp-α1

bait

VSV tag

tim 

tim

prey

V5 tag

tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
NLS domain
mutation disrupting interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jang et al., 2015)

A mutation in the NLS domain of tim prevents binding to Kap-α1.

(Jang et al., 2015)
FBrf0227552-7.coIP.WB
Reference
Jang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Kap-α1 

imp-α1

bait

VSV tag

tim 

tim

prey

V5 tag

tim 
tim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tim 
interacting residue 155
mutation disrupting interaction
aa 155

P155L

tim 
interacting residue 113
mutation disrupting interaction
aa 113

T113A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutations in tim at residues P155L or T113 prevent binding to Kap-α1.

(Jang et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jang et al., 2015)
FBrf0227552-8.coIP.WB
Reference
Jang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Kap-α1 

imp-α1

bait

HA tag

tim 

tim

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Expression driven by TUG-GAL4.

(Jang et al., 2015)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jang et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)