Source was 293T cells; proteins produced from transfected constructs.

upd1 binding specifically to cells expressing dome at the cell surface was taken as evidence of a upd1-dome interaction.

dome, but not et, can interact with upd1, upd2 and upd3 ligands.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Receptor and ligand were co-transfected into the same cells (autocrine ligand binding assay).