FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103871
Interaction Network
Interactions Browser links
brk network
gro network
Reported Interactions
FBrf0139645-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brk 

Brk

bait

GST tag

brk 
brk 
gro 

Groucho

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brk 
repression domain
sufficient binding region
aa 369-541

"RD" construct

brk 
FKPY motif
mutation disrupting interaction
FKPY

KP to EA mutation, "mutG" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0139645-5.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brk 

Brk

bait

fused to DNA-binding domain

brk 
brk 
gro 

Gro

prey

fused to activation domain

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brk 
repression domain
sufficient binding region
aa 369-541

"RD" construct

brk 
FKPY motif
mutation disrupting interaction
FKPY

KP to EA mutation, "mutG" construct

gro 
region
sufficient binding region
aa 251-719

"pZP54" construct, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast

FBrf0174967-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brk 

Brk

bait

GST tag

gro 

Gro

prey

[35]S label

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
interaction domain
necessary binding region
aa 175-719

coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait source unspecified; prey produced and labeled by in vitro translation.

FBrf0207459-1.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brk 

Brk

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0217447-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brk 

Brk

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)