Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.
There were no differences in interaction between extracts produced from a transgenic fly line expressing wild-type pim or pim with a mutated D and Ken box.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
unspecified substitution of aa C88
unspecified substitution of aa L90
unspecified substitution of aa Y93
unspecified substitution of aa E98
unspecified substitution of aa N103
unspecified substitution of aa F105
unspecified substitution of aa Y107
unspecified substitution of aa F110
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type larval brain extract.
