FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103933
Interaction Network
Interactions Browser links
kni network
CtBP network
Reported Interactions
FBrf0101970-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kni 

Knirps

bait

fused to GAL4 DNA-binding domain

kni 
CtBP 

dCtBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kni 
repression domain
sufficient binding region
aa 255-429

coordinates relative to kni-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0101970-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

kni 

knirps

prey

[35]S label

kni 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kni 
P-DLS-K motif
mutation disrupting interaction
DLS

mutated to AAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

383aa isoform of CtBP used for pull down.

FBrf0220212-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

kni 

Knirps

prey

[35]S label

kni 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kni 
PXDLS motif
mutation decreasing interaction
aa 331-334

PMDLS to AAAAS, coordinates relative to kni-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)