FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pum, brat
FlyBase ID
FBig0000104008
Interaction Network
Interactions Browser links
pum network
brat network
Reported Interactions
FBrf0135683-4.Y4H
Reference
Edwards et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

fused to GAL4 DNA-binding domain

pum 
pum 
pum 
pum 
pum 
brat 

Brat

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
Puf domain
sufficient binding region
aa 1092-1411

coordinates relative to pum-PA

pum 
Puf domain residue G1330
mutation disrupting interaction
aa 1330

G1330D, coordinates relative to pum-PA

pum 
Puf domain residue C1365
mutation disrupting interaction
aa 1365

C1365R, coordinates relative to pum-PA

pum 
Puf domain residue T1366
mutation disrupting interaction
aa 1366

T1366D, coordinates relative to pum-PA

pum 
Puf domain residue N1368
mutation disrupting interaction
aa 1368

N1368S, coordinates relative to pum-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Edwards et al., 2001)

Four-hybrid system: yeast GAL4-BD/GAL4-AD including pum-GAL4-BD, brat-GAL4-AD, hb NRE RNA and nos protein.

(Edwards et al., 2001)
FBrf0135777-2.Y4H
Reference
Sonoda and Wharton, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

fused to GAL4 DNA-binding domain

pum 
brat 

Brat

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RNA binding domain
sufficient binding region
aa 1093-1426

coordinates relative to pum-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Sonoda and Wharton, 2001)

Four-hybrid system: yeast GAL4-BD/GAL4-AD including pum-GAL4-DBD, nos protein and nos RNA (NRE site) intermediates, and prey fused to GAL4 activation domain.

(Sonoda and Wharton, 2001)
FBrf0135777-6.PD.A
Reference
Sonoda and Wharton, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

GST tag

pum 
brat 

Brat

prey

His tag

brat 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RNA binding domain residue G1330
mutation disrupting interaction
aa 1330

G1330D, coordinates relative to pum-PA

brat 
NHL domain
sufficient binding region
aa 736-1037

coordinates relative to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sonoda and Wharton, 2001)
FBrf0193740-4.PD.WB
Reference
Cho et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

GST tag

pum 
brat 

Brat

prey

His tag

brat 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RNAB domain
sufficient binding region

coordinates not reported

brat 
NHL domain
sufficient binding region

coordinates not reported

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Cho et al., 2006)

Recombinant GST-pum was mixed with hb RNA (NRE) and nos, brat and eIF4EHP proteins in various combinations.

(Cho et al., 2006)
FBrf0194692-4.PD.WB
Reference
Kadyrova et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

GST tag

brat 

Brat

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Kadyrova et al., 2007)
FBrf0224581-5.CH.MW
Reference
Loedige et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

brat

neutral component
brat 

brat

neutral component
brat 
brat 

brat

neutral component
brat 
pum 

pum

neutral component
pum 
pum 

pum

neutral component
pum 

pum

neutral component
pum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brat 
NHL domain
sufficient binding region
aa 756-1037

coordinates refer to brat-PA

pum 
HD domain
sufficient binding region
aa 1093-1426

coordinates refer to pum-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Loedige et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)