mud was present in low abundance in S2 cells, and its levels were not influenced by the amount of ana2 or ctp. However, increased amounts of Mud protein were present in the immune complex with HA-ctp when Myc-ana2 was coexpressed, suggesting that increasing ctp recruited more mud. Increasing amounts of ana in S2 cells also appeared to stabilize ctp. into the protein complex.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

mud was present in low abundance in S2 cells, and its levels were not influenced by the amount of ana2 or ctp. However, increased amounts of Mud protein were present in the immune complex with HA-ctp when Myc-ana2 was coexpressed, suggesting that increasing ctp recruited more mud into the protein complex.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Size of complex suggests 4 ana2 molecules and 8 ctp molecules.