Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Live S2 cells were incubated with anti-FLAG and anti-Myc to label only cell surface localized proteins. Cells were then fixed, and signal developed using proximity ligation assay in which a signal develops only both primary antibodies are within tens of nanometers.
Source was fixed S2 cell line; proteins produced from transfected construct.
Live tissue was incubated with anti-FLAG and anti-Myc to label only cell surface localized proteins. Tissue was then fixed, and signal developed using proximity ligation assay in which a signal develops only both primary antibodies are within tens of nanometers.
Source was fixed larval body wall; proteins produced from tagged transgenic construct.
Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Addition of PhTx increases interaction.
Source was larvae of transgenic fly line; E(bx) produced from tagged transgenic construct; dlg1 produced from endogenous gene.
coordinates relative to mys-PA
Source was larvae of transgenic fly line; E(bx) produced from tagged transgenic construct; dlg1 produced from endogenous gene.
Addition of PhTx increases interaction with wild-type mys.
