FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ash1, fs(1)h
FlyBase ID
FBig0000104125
Interaction Network
Interactions Browser links
ash1 network
fs(1)h network
Reported Interactions
FBrf0231041-1.coIP.MS
Reference
Kockmann et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
ash1 

ASH1

bait

FLAG tag, His tag

fs(1)h 

fs(1)h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kockmann et al., 2013)

Bait protein was isolated from nuclear extracts via the His tag (by immobilized metal affinity chromatography), then further purified by ion exchange chromatography before FLAG affinity chromatography.

(Kockmann et al., 2013)
FBrf0231041-2.coIP.WB
Reference
Kockmann et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
ash1 

ASH1

bait

FLAG tag, GFP tag

ash1 
fs(1)h 

fs(1)h

prey

HA tag

fs(1)h 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ash1 
N-terminal region
sufficient binding region
aa 1-763
fs(1)h 
N-terminal region
sufficient binding region
aa 1-1110

coordinates relative to fs(1)h-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kockmann et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)