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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104134
Interaction Network
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ato network
da network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0057896-2.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
da 

da

neutral component
da 

da

neutral component
da 

da

neutral component
ato 

ato

neutral component
ato 

ato

neutral component
ato 

ato

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A [32]P labeled DNA probe consisting of an E box was mixed with ato and/or da. Cooperative binding to the DNA was observed by EMSA.

da and ato bind cooperatively to DNA. For the DNA probe tested, neither da nor ato could bind alone.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

FBrf0174564-1.coIP.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
da 

DA

bait

Myc tag

ato 

ATO

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Protein sources unspecified.

FBrf0184217-10.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
da 

Da

bait

GST tag

ato 

Ato

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0211413-1.EMSA.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
da 

da

neutral component

His tag

da 

da

neutral component

His tag

da 

da

neutral component

His tag

ato 

ato

neutral component

His tag

ato 

ato

neutral component

His tag

ato 

ato

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

da and ato were mixed with [32]P labeled DNA and DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions. Neither binds DNA on its own in this assay.

FBrf0230799-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ato 

Ato

bait

fused to LexA DNA-binding domain

ato 
da 

Da

prey

fused to GAL4 activation domain

da 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ato 
bHLH domain
sufficient binding region
aa 252-312
da 
C-terminal region
sufficient binding region
aa 539-662
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The ato-da interaction is not affected by mutation of ato\'s protein kinase A phosphorylation site (S292A and S292D tested): wild-type, phospho-mimetic and phospho-null ato heterodimerize with da.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)