Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Whamy binds to GTP-bound Rac1. Only one of the two Whamy CRIB domains is required for binding, but deletion of both CRIB domains abolishes the interaction.

Whamy and WASp colocalize at vesicles in these experiments.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.