Mutation of mir-14 increases sug mRNA levels.

Source was adult heads of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

GFP reporter assay

Source was adult brains of mutant fly line; bait produced from endogenous gene; prey produced from transgenic reporter construct.

Mutation of mir-14 increases sug GFP reporter expression in insulin producing cells.

luciferase reporter assay

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.