FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nedd4, SH3PX1
FlyBase ID
FBig0000104345
Interaction Network
Interactions Browser links
Nedd4 network
SH3PX1 network
Reported Interactions
FBrf0231307-4.PD.MS
Reference
Safi et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

dNedd4Lo

bait

GST tag

Nedd4 
Nedd4 
SH3PX1 

SH3PX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nedd4 
middle region
sufficient binding region
aa 304-473

coordinates relative to Nedd4-PJ

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Safi et al., 2016)
FBrf0241343-1.coIP.WB
Reference
Wasserman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

dNedd4

bait

FLAG tag

Nedd4 
Nedd4 
SH3PX1 

SH3PX1

prey

HA tag

SH3PX1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SH3PX1 
SH3 domain
mutation decreasing interaction
unspecified

deletion of SH3 domain

Nedd4 
Mid domain residues 392,395,398
mutation decreasing interaction
aa 392,395,398

REAPGVP to AEAAGVA, coordinates relative to Nedd4-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wasserman et al., 2019)
FBrf0241343-2.PD.WB
Reference
Wasserman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

dNedd4

bait

GST tag

Nedd4 
SH3PX1 

SH3PX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nedd4 
Mid domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Wasserman et al., 2019)
FBrf0241343-3.PD.WB
Reference
Wasserman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nedd4 

dNedd4

bait

FLAG tag

Nedd4 
SH3PX1 

SH3PX1

prey

His tag

SH3PX1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nedd4 
Mid domain
sufficient binding region
unspecified
SH3PX1 
SH3 domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Wasserman et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)