FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104355
Interaction Network
Interactions Browser links
bru1 network
Reported Interactions
FBrf0227674-1.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bru1 

Bru

bait

GST tag

bru1 

Bru

prey

His6 tag

bru1 
bru1 
bru1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bru1 
interaction domain 1
sufficient binding region
aa 1-146
bru1 
interaction domain 1
necessary binding region
aa 1-146
bru1 
interaction domain 1
mutation disrupting interaction
aa 1-146
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0227674-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bru1 

Bru

bait

GST tag

bru1 

Bru

prey

His6 tag

bru1 
bru1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bru1 
interacting amino acid 7
mutation decreasing interaction
aa 7

S7E, in the presence of S4E mutation, completely disrupts the interaction.

bru1 
interacting amino acid 4
mutation disrupting interaction
aa 4

S4E requires the mutation S7E to disrupt the interaction.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Amino acid mutation S7E of aret decreases the interaction. Both S4E and S7E together completely disrupt the interaction.

External Crossreferences and Linkouts ( 0 )
References (1)