Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
S7E, in the presence of S4E mutation, completely disrupts the interaction.
S4E requires the mutation S7E to disrupt the interaction.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Amino acid mutation S7E of aret decreases the interaction. Both S4E and S7E together completely disrupt the interaction.