Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
L70A, coordinates relative to flfl-PD
F92A,P95A, coordinates relative to mira-PB
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
