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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104485
Interaction Network
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p53 network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210534-1.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

MBP tag

p53 

Dmp53

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey produced and labeled by in vitro translation.

FBrf0229995-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

p53B

bait

Ch tag

p53 

p53A

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Self-interaction via two isoforms.

Source was wing disc of third instar larva of transgenic fly; bait produced from transgenic gene; prey produced from transgenic gene.

FBrf0243175-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

p53A

bait
p53 

p53B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

An antibody specific to the p53-PA isoform immunoprecipitates the p53-PB isoform as well suggesting p53-PA/p53-PB hetero-oligomerization.

External Crossreferences and Linkouts ( 0 )
References (3)