FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4A, raptor
FlyBase ID
FBig0000104497
Interaction Network
Interactions Browser links
eIF4A network
raptor network
Reported Interactions
FBrf0232307-4.coIP.WB
Reference
Tsokanos et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait

FLAG tag

raptor 

Raptor

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The RagC-D-eIF4G interaction is observed only upon incubation with DSP crosslinker, or co-transfection of RagA-B.

(Tsokanos et al., 2016)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsokanos et al., 2016)

Proteins were crosslinked with DSP prior to immunopreciptation.

(Tsokanos et al., 2016)
FBrf0232307-8.coIP.WB
Reference
Tsokanos et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait

FLAG tag

raptor 

Raptor

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsokanos et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)