FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-7, ihog
FlyBase ID
FBig0000104498
Interaction Network
Interactions Browser links
mir-7 network
ihog network
Reported Interactions
FBrf0221556-1.miR-mRNA.LUC
Reference
Da Ros et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-7 

mir-7

bait
mir-7 
ihog 

ihog

prey
ihog 
ihog 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ihog 
3-prime UTR
sufficient binding region
ihog 
mir-7 target site
necessary binding region
GUCCAUUCUACGCUAAGUCUUCCA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

luciferase reporter assay

(Da Ros et al., 2013)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Da Ros et al., 2013)
FBrf0221556-2.miR-mRNA.GFP
Reference
Da Ros et al., 2013
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-7 

mir-7

bait
mir-7 
ihog 

ihog

prey
ihog 
ihog 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ihog 
3-prime UTR
sufficient binding region
ihog 
mir-7 target site
necessary binding region
GUCCAUUCUACGCUAAGUCUUCCA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GFP reporter assay

(Da Ros et al., 2013)

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Da Ros et al., 2013)
FBrf0221556-3.miR-mRNA.PCR
Reference
Da Ros et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-7 

mir-7

bait
ihog 

ihog

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-7 reduces ihog mRNA levels.

(Da Ros et al., 2013)

Source was larvae of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Da Ros et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)