FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hat1, His4
FlyBase ID
FBig0000104502
Interaction Network
Interactions Browser links
Hat1 network
His4 network
Reported Interactions
FBrf0231314-15.coIP.MS
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hat1 

CG2051

bait

Strep tag, FLAG tag

His4 

H4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)
FBrf0231314-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hat1 

HAT1

enzyme

GST tag

Hat1 

HAT1

enzyme

GST tag

His4 

H4

enzyme target

C[14] label

His4 

H4

enzyme target

C[14] label

His4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0231314-22.coIP.WB
Reference
Boltengagen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hat1 

CG2051

bait

FLAG tag

His4 

H4K5Ac

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CG2051 interacts with Caf1-55, cid and H4K5Ac-modified His4 in this fraction, but not cal1, dre4 or Ssrp.

(Boltengagen et al., 2016)

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Boltengagen et al., 2016)

A gel filtration fraction ("B15") containing complexes of about 180 kDa was used for immunoprecipitation.

(Boltengagen et al., 2016)
External Crossreferences and Linkouts ( 0 )
References (1)