FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fs(1)h, Pc
FlyBase ID
FBig0000104529
Interaction Network
Interactions Browser links
fs(1)h network
Pc network
Reported Interactions
FBrf0213360-19.PD.MS
Reference
Strübbe et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

BioTag

fs(1)h 

Fs(1)h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between the bait and BioTag

(Strübbe et al., 2011)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Strübbe et al., 2011)
FBrf0213360-26.PD.WB
Reference
Strübbe et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

BioTag

fs(1)h 

Fs(1)h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between the bait and BioTag

(Strübbe et al., 2011)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Strübbe et al., 2011)
FBrf0228678-7.TAP.MS
Reference
Kang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

BioTAP tag

fs(1)h 

fs(1)h

prey

75 peptide counts

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kang et al., 2015)
FBrf0250608-1.coIP.WB
Reference
Lv et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fs(1)h 

Fsh

bait

Flag tag

fs(1)h 
Pc 

Pc

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fs(1)h 
interaction domain
sufficient binding region
aa 868-1110
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

2 round coIP: fs(1)h-FLAG immunoprecipitated Pc and Sce in the first round, and Pc-V5 immunoprecipitated fs(1)h-FLAG and Sce in the second round, indicating a complex of all three.

(Lv et al., 2018)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lv et al., 2018)
FBrf0250608-3.coIP.WB
Reference
Lv et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

V5 tag

fs(1)h 

Fsh

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lv et al., 2018)

2 round coIP: fs(1)h-FLAG immunoprecipitated Pc and Sce in the first round, and Pc-V5 immunoprecipitated fs(1)h-FLAG and Sce in the second round, indicating a complex of all three.

(Lv et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)