FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dbr, ci
FlyBase ID
FBig0000104668
Interaction Network
Interactions Browser links
dbr network
ci network
Reported Interactions
FBrf0160468-1.Y2H
Reference
Dai et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

fused to LexA DNA-binding domain

ci 
dbr 

Dbr

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal region
sufficient binding region
aa 1-441

coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

17 of 100 positives in the screen for ci-interactors correspondeded to dbr.

(Dai et al., 2003)

Two-hybrid system: yeast LexA-BD/AD

(Dai et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Dai et al., 2003)
FBrf0160468-2.coIP.A
Reference
Dai et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
dbr 

Dbr

bait

FLAG tag

ci 

Ci

prey

[35]S label

ci 
ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal repressor domain
sufficient binding region
aa 1-440

moderate binding, coordinates relative to ci-PA

ci 
Zinc finger domain
sufficient binding region
aa 441-683

strong binding, coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait immunopurified from transfected CME-W1-Cl.8+ cell extract; prey produced and labeled by in vitro translation.

(Dai et al., 2003)
FBrf0160468-3.PD.A
Reference
Dai et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

GST tag

ci 
ci 
dbr 

Dbr

prey

[35]S label

dbr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
N-terminal repressor domain
sufficient binding region
aa 1-440

moderate binding, coordinates relative to ci-PA

ci 
Zinc finger domain
sufficient binding region
aa 441-683

strong binding, coordinates relative to ci-PA

dbr 
N-terminal region
sufficient binding region
aa 1-243

coordinates relative to dbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Dai et al., 2003)

The dbr N-terminal region binds, independently, to both the N-terminal repressor and Zinc finger domains of ci.

(Dai et al., 2003)
FBrf0160468-4.coIP.WB
Reference
Dai et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
dbr 

Dbr

bait
ci 

Ci

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dai et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)